Method for producing alpha-L-Aspartyl-L-phenylalanine-Beta-ester and method for producing alpha-L-aspartyl-L-phenylalanine-alpha-methyl ester

摘要

A method for producing an ±-L-aspartyl-L-phenylalanine-²-ester (also named ±-L-(²-o-substituted aspartyl)-L-phenylalanine), which is an intermediate of an ±-L-aspartyl-L-phenylalanine-²-methyl ester (also named ±-L-aspartyl-L-phenylalanine methyl ester; product name: aspartame), easily, at high yield and inexpensively without going through a complex synthesis method is provided. Further, an easy, inexpensive and high-yield production method for an ±-L-aspartyl-L-phenylalanine-±-methyl ester is provided. ±-L-aspartyl-L-phenylalanine-±-methyl ester is produced from a L-aspartic acid-±,²-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to catalyze a reaction in which L-phenylalanine performs no nucleophilic attack on a ²-ester site of L-aspartic acid-±,²-diester but performs a nucleophilic attack on an ±-ester site thereof.