发明名称 |
METHOD FOR EVALUATING FLUORESCENCE RESULTS IN A MICROSCOPE IMAGE |
摘要 |
The invention allows a quantitative evaluation of images acquired by microscope having fewer errors and is applicable in connection with high-resolution methods, particular at a high speed. A microscope image is analyzed in which the intensity distributions of fluorescence events have in each instance a diffraction-dependent extent which corresponds to an extent of a point spread function of the microscope and are arranged so as to be spatially non-overlapping, or at least predominantly spatially non-overlapping, in that at least one counter is initialized for every region to be analyzed in the microscope image, at least one fluorescence event is identified in a region to be analyzed in the microscope image, and the counter corresponding to the relevant region is incremented for each fluorescence event identified in the region. The counting results in a dramatic improvement in the signal-to-noise ratio at a high evaluation speed. |
申请公布号 |
EP2446314(A1) |
申请公布日期 |
2012.05.02 |
申请号 |
EP20100728594 |
申请日期 |
2010.06.19 |
申请人 |
CARL ZEISS MICROIMAGING GMBH |
发明人 |
KLEPPE, INGO;KALKBRENNER, THOMAS;WOLLESCHENSKY, RALF |
分类号 |
G02B21/00;G01N21/64 |
主分类号 |
G02B21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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