IMPROVED SYNTHESIS OF BIOSYNTHETIC PRODUCT BY ORDERED ASSEMBLY OF BIOSYNTHETIC ENZYMES GUIDED BY THE NUCLEOTIDE SEQUENCE MOTIF TEMPLATE

摘要

Invention refers to a method producing a compound by a biosynthetic pathway by culturing a genetically modified host cell, wherein the cell is modified with one or more nucleic acids comprising nucleotide sequence encoding chimeric proteins composed of biosynthetic pathway enzyme or other functional polypeptide and nucleic acid binding factor and program nucleic acid sequence that contains target nucleic acid elements that are recognized by said nucleic acid binding factor from the chimeric protein and where the said program nucleic acid sequence provides arrangement of biosynthetic pathway enzymes or other functional polypeptides along the program nucleic acid sequence and said culturing providing for synthesis of said chimeric proteins in the genetically modified host cells resulting in a production of said compound. Invention refers to host cells with program nucleic acid sequence, nucleic acid sequence expressing chimeric proteins. Invention provides methods improving product synthesis.