The present invention provides a method for identifying differential activation of a bisubstrate protein modifying enzyme between samples, comprising: (i) incubating a first sample with x different concentrations of the non-protein substrate of said enzyme, wherein x is 2 or greater than 2; (ii) quantifying modification of a polypeptide in said sample at each of the x different concentrations of the non-protein substrate; (iii) determining the affinity of said enzyme for said non-protein substrate; (iv) repeating steps (i) to (iii) for a second or subsequent sample; and (v) comparing the affinity of said enzyme for said non-protein substrate between said samples; wherein a difference in affinity of said enzyme for said non-protein substrate between samples is indicative of differential activation of said enzyme between samples. The present invention also provides a method for identifying an in vivo substrate of a bisubstrate protein modifying enzyme.
申请公布号
WO2012052711(A1)
申请公布日期
2012.04.26
申请号
WO2011GB01497
申请日期
2011.10.18
申请人
QUEEN MARY & WESTFIELD COLLEGE;RODRIGUEZ CUTILLAS, PEDRO;VANHAESEBROECK, BART;BELTRAN, LUISA, MARIE
发明人
RODRIGUEZ CUTILLAS, PEDRO;VANHAESEBROECK, BART;BELTRAN, LUISA, MARIE