STATE DETERMINATION METHOD FOR CELL CLUSTER, IMAGE PROCESSING PROGRAM AND IMAGING PROCESSING DEVICE USING SAID METHOD, AND METHOD FOR PRODUCING CELL CLUSTER

摘要

Provided is means by which the state of change of a cell aggregation toward becoming multi-layered can be decided from two observation images taken at a predetermined time interval. An image processing program (GP) is provided with a step (S10) for obtaining a first image and a second image taken by an imaging device at a predetermined time interval; a step (S40) for performing block matching, using the luminance distribution of a local region of the first image as a standard, in a vicinity including the corresponding position of the second image, taking the degree of approximation of the region with the highest degree of matching as a representative degree of approximation, sequentially moving the local region, and calculating the representative degree of approximation for individual parts of the cell aggregation; and a step (S50) for outputting multi-layering information corresponding to the calculated representative degree of approximation. The image processing program (GP) is configured so as to output multi-layering information by which the state of change of the cell aggregation toward becoming multi-layered can be decided from the first and second images.