发明名称 |
Use of toll-like receptor-expressing cells |
摘要 |
Swine Toll-like receptor 9 (TLR9)-expressing cells are prepared by cloning a TLR9 gene from swine intestinal Peyer's patches. Functional analysis on CpG DNAs using the above cells revealed that swine TLR9 shows a higher recognition ability for a human CpG DNA motif (CpG2006) than for a mouse-specific CpG DNA motif (CpG1826). When the mRNA expression levels in various tissues are compared by the real-time PCR method, it is found out that the mRNA is expressed in Peyer's patches and mesenteric lymph nodes, which play important roles in the intestinal tract immune system, at a level thrice as much as in spleen or more. Thus, the cells expressing an intestinal tract tissue-expressed TLR (for example, TLR9) can be used to identify samples capable of activating the intestinal tract immune system.
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申请公布号 |
US8163506(B2) |
申请公布日期 |
2012.04.24 |
申请号 |
US20040561022 |
申请日期 |
2004.03.05 |
申请人 |
SAITO TADAO;SHIMOSATO TAKESHI;KITAZAWA HARUKI;MEIJI CO., LTD. |
发明人 |
SAITO TADAO;SHIMOSATO TAKESHI;KITAZAWA HARUKI |
分类号 |
G01N33/567;C07K14/705;C12N15/117;G01N33/50 |
主分类号 |
G01N33/567 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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