发明名称 |
METHOD FOR PRODUCING TRANSGENIC ANIMAL MODELS WITH MODULATED PHENOTYPE AND ANIMALS PRODUCED THEREFROM |
摘要 |
A method of preparing a transgenic animal model with enhanced, accelerated pathology for Alzheimer's Disease (AD) and the transgenic animal made by the method are disclosed. The method includes producing an F1 generation by crossing a first and second transgenic parent each carrying a different expressible transgene for differing aspects of the same desired phenotype associated with AD pathology. The offsprings of the F1 generation are then screened and those which carry a transgene from each parental transgenic animal resulting in an enhanced pathology for Alzheimer's Disease are selected. In a preferred embodiment the AD-associated pathology is for amyloid accumulation. In an embodiment a mutant presenilin transgene and a transgene for a mutant amyloid precursor protein are used. In a further embodiment the mutant presenilin transgene is the PS1 M146L mutation and the mutant amyloid precursor protein transgene is the Swedish mutation (app695 isoform containing a K670N, M671L mutation (app770 numbering )).
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申请公布号 |
CA2268812(C) |
申请公布日期 |
2012.04.24 |
申请号 |
CA19972268812 |
申请日期 |
1997.10.15 |
申请人 |
UNIVERSITY OF SOUTH FLORIDA |
发明人 |
DUFF, KAREN;HARDY, JOHN |
分类号 |
A01K67/027;C12N5/00;A61K49/00;C07K14/47;C12N15/00;C12N15/09;C12N15/85;C12Q1/68 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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