METHODS FOR PRODUCING HAIR MICROFOLLICLES AND DE NOVO PAPILLAE AND THEIR USE FOR IN VITRO TESTS AND IN VIVO IMPLANTATIONS

摘要

The present invention relates to a method for producing de novo papillae, in which dermal hair papilla fibroblasts (DPFs) are isolated from a dermal papilla (DP) of a mammal hair follicle, subsequently expanded, and finally condensed into cell aggregates that exhibit the size and shape of the physiological DP, wherein said DPFs are differentiated in ultra-low attachment culture vessels in a cell concentration per vessel surface of 1.000 to 100.000 DPFs/cm 2 . The invention also relates to a method for producing hair microfollicles by co-culturing the de novo papillae with another cell population of the hair follicle in the ultra-low attachment culture vessels. Object of the invention are also the de novo papillae and the hair microfollicles produced by the aforementioned methods. The de novo papillae and hair microfollicles can be used as implants for treating reduced hair conditions, and for in vitro testing hair-modulating effects or toxic effects of substances.