| 摘要 |
An immiscible mixture of wax and silicone oil is provided for application to the inner surface of a thermocycle reactor tube. The mixture is a solid at typical room temperatures and under typical product storage conditions, but at temperatures above the melting point of the mixture (e.g., > 46° C and therefore, at or above 90° C (which is typical of the first stage of a PCR thermocycle)) the mixture melts and covers the exposed surface of the solution undergoing the thermocycle, thereby sealing the reaction against evaporation and/or condensation over the duration of the multiple thermocycles typical of a PCR reaction. Upon cooling of the reaction tube, the resulting amplicons can then extracted from below the sealing layer. |
