ANTIGEN FOR HEMAGGLUTINATION OF RABBIT HAMORRHAGIC DISEASE VARIANT VIRUS USING RECOMBINANT VP60 BACULOVIRUS AND METHOD OF RECOMBINANT INACTIVATED VACCINE

摘要

PURPOSE: A method for preparing an antigen for hemagglutination of rabbit hemorrhagic disease variant virus and recombinant inactivated vaccine is provided to reduce biological risk. CONSTITUTION: An antigen for suppressing hemagglutination is prepared by inoculating recombinant baculovirus prepared from VP60 gene(deposit number: KCTC 11756BP) of rabbit hemorrhagic disease variant virus into tissue culture cells. The tissue cultured cell is an insect cell(sf-9cell). A method for manufacturing an recombinant inactivated vaccine comprises: a stepo f cloning VP60 genes into pGEM-T vector; a step of cloning to pBuleBacHis4.5 vector to prepare recombinant baculovirus; a step of inoculating the baculovirus into the insect cells to prepare VP gene recombinant protein; a step of isolating antigen from the recombinant protein; and a step of inactivating the antigen.