| 摘要 |
The present invention in general provides a method for amplification of a target DNA, comprising the steps of (i) transfering a liquid with a first volume comprising at least one or more living cells into a vessel (ii) adding to said vessel a PCR reaction buffer with a second volume, whereas said second volume is at least 2x as large as said first volume (iii) lysing said at least one or more living cells within said vessel by means of incubation for at least 1 Minute at at least 90°C, and (iv) amplifying said target by means of a polymerase chain reaction without performance of an intermediate purification step. |
