Provided is a method of identifying an unknown nucleic acid comprising the steps of combining the unknown polynucleic acid with known nucleic acid reagents in a reaction chamber; producing a first quantity of protons from a polymerisation reaction when bases of one or more of the unknown nucleic acids are complementary to the bases of one or more known nucleic acids comprised within the known reagents; producing a second quantity of protons from a hydrolysis reaction of by-products of the polymerisation reaction with one or more enzymes; monitoring an electrical output signal derived from an ISFET exposed to the reaction chamber; and correlating changes in an output signal with said reactions between the unknown polynucleic acid and said known reagents to thereby identify the unknown nucleic acid.
申请公布号
WO2012045889(A1)
申请公布日期
2012.04.12
申请号
WO2011EP67661
申请日期
2011.10.10
申请人
DNA ELECTRONICS LTD;OU, CHUNG PEI;WANG, CHAN JU;MORLEY, DANIEL;REED, SAM
发明人
OU, CHUNG PEI;WANG, CHAN JU;MORLEY, DANIEL;REED, SAM