摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for measuring hemoglobins capable of measuring hemoglobins in a short time with high precision and capable of enhancing the life of a column. <P>SOLUTION: In a method for measuring hemoglobins by liquid chromatography in which eluent is delivered to a column packed with a column filler, particles, which comprise a polymer which is composed of 10 to 200 pts.wt. of a monomer containing one or more sulfonic acid groups in a molecule, 5 to 100 pts.wt. of a cross-linkable monomer having no sulfonic acid group and having one or more hydroxyl groups in a molecule and 100 pts.wt. of a cross-linkable monomer having neither sulfonic acid group nor hydroxyl group as constitutional units, are used as the column filler, and the pressure value occurred in a measurement system is set at 9.8×10<SP POS="POST">3</SP>Pa or more and less than 9.8×10<SP POS="POST">4</SP>Pa. <P>COPYRIGHT: (C)2012,JPO&INPIT |