| 摘要 |
<p>Method for prepn. of vaccines (I). A multiple emulsion is prepared in which an aqueous medium containing the antigen(s), is dispersed in an oil (e.g. Drakeol 6VR, Bayol F, or Arachis) with the aid of an emulsifying agent (5-15% Arlacel A), the particle size being 1-7 mu. This is then dispersed in a second aqueous phase, the ratio (oil:water) being between 3:1 and 1:10, by the use of a suitable emulsifying agent (e.g.Tween 80), and a homogeniser, or ultra-sonic vibration (18000-20000 Hz). The size of the oil particles is 10-50 mu. A more stable emulsion is produced by the addition of 1% agar. Any type of antigen may be used, including influenza virus, Newcastle disease virus, tetanus toxoid, polio virus, Bordetella pertussis bacteria, measles virus, and diptheria toxoid. (1) Low viscosity (2) I is rapidly transported through the body (3) Multiple dose containers can be used.</p> |
