发明名称 |
METHOD FOR PRODUCING NATIVELY FOLDED PROTEINS IN A PROKARYOTIC HOST |
摘要 |
The present invention relates to a method for producing a protein of interest containing one or more disulfide bonds in its native state. The method comprises that a prokaryotic host cell is genetically engineered to express the protein of interest and a sulfhydryl oxidase in the cytoplasm of the host cell. The protein of interest is formed in a soluble form and contains disulfide bonds due to the presence of the sulfhydryl oxidase in the cytoplasm of said host cell. The present invention relates also to a prokaryotic host cell and a vector system for producing a protein of interest containing natively folded disulfide bonds. |
申请公布号 |
EP2438175(A1) |
申请公布日期 |
2012.04.11 |
申请号 |
EP20100726551 |
申请日期 |
2010.06.02 |
申请人 |
OULUN YLIOPISTO |
发明人 |
RUDDOCK, LLOYD |
分类号 |
C12N15/70;C07K14/00;C12N5/00;C12N9/00;C12N9/02;C12N15/00 |
主分类号 |
C12N15/70 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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