USE OF AN ENDOGENOUS 2-MICRON YEAST PLASMID FOR GENE OVER EXPRESSION
摘要
<p>Methods and compositions for making stable recombinant yeast 2 µm plasmids are provided. Homologous recombination is performed to clone a nucleic acid of interest into the yeast 2 µm plasmid. Heterologous nucleic acid subsequences are recombined between an FLP and a REP2 gene of the plasmid.</p>
