| 摘要 |
<P>PROBLEM TO BE SOLVED: To rapidly detect enterovirus 71 RNA in high sensitivity. <P>SOLUTION: The method for detecting the enterovirus 71 RNA includes a step of measuring the amount of an amplified RNA product with a nucleic acid probe designed so that the signal characteristics may be changed when a complementary double strand is formed with the amplified RNA, in an RNA amplification step comprising a step of using a combination of oligonucleotides comprising a first primer hybridizing with a specific base sequence in the enterovirus 71 RNA under a stringent condition and a second primer (one of the primers has a promoter sequence added to the 5' terminus), forming a double-stranded DNA containing the promoter sequence by a reverse transcriptase, forming an RNA transcription product by using the double-stranded DNA as a template by an RNA polymerase, and forming the double-stranded DNA by consecutively using the RNA transcription product as the template of DNA synthesis by the reverse transcriptase. <P>COPYRIGHT: (C)2012,JPO&INPIT |
