发明名称 |
COMPOSITIONS AND METHODS FOR EXPANDING BFU-E CELLS |
摘要 |
The invention provides methods of expanding BFU-E cells comprising contacting one or more BFU-E cells with a hypoxia inducible factor 1 activator and a glucocorticoid receptor (GR) activator, e.g., a GR agonist. In some embodiments, the FIIF-1 activator is a prolyl hydroxylase inhibitor (PHI). In some embodiments, the method comprises culturing BFU-E in medium containing a HIF-la activator and a glucocorticoid receptor (GR) activator, e.g., a GR agonist. In some embodiments the BFU-E cells are human cells. The invention provides cell culture media useful for expanding BFU-Es, wherein the cell culture media comprise a HIF-1 activator and a GR activator (e.g., a GR agonist) The invention provides a method comprises administering a HIF-1 activator and a GR agonist to a subject in need thereof. In some embodiments, the subject suffers from anemia. In some embodiments, the anemia is an Epo-resistant anemia. In some embodiments, the anemia is Diamond-Blackfan anemia. |
申请公布号 |
WO2011113036(A3) |
申请公布日期 |
2012.04.05 |
申请号 |
WO2011US28248 |
申请日期 |
2011.03.12 |
申请人 |
WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH;FLYGARE, JOHAN;LODISH, HARVEY, F. |
发明人 |
FLYGARE, JOHAN;LODISH, HARVEY, F. |
分类号 |
C12N5/078;A61K31/56;A61K38/00;A61P7/06;C12N5/02 |
主分类号 |
C12N5/078 |
代理机构 |
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代理人 |
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地址 |
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