| 摘要 |
The present invention discloses a method of visualizing the 3-dimensional microstructure of a thick biological tissue. This method includes: a process of immersing thick, opaque biological tissues in the optical-clearing solution, for example FocusClear (U.S. Pat. No. 6,472,216), and utilizing an optical scanning microscope and a cutter. In microscopy, the cutter removes a portion of the tissue after each round of optical scanning. Each round of optical scanning follows the principal that the depth of the removal plane is less than the depth of the boundary plane derived from the scanning This method acquires an image stack to provide the information of thick biological tissue's 3-dimensional microstructure with minimal interference by the tissue removal. |
