Relative quantification analysis of multi-parametric PCR experiments

摘要

An analysis method for quantitative PCR experiments comprising multiple genes of interest, multiple samples and multiple conditions is provided. It was found that for calculating the relative expression status of multiple target genes from multiple samples under multiple different conditions, a calculation method based on up to three different parameters has to be performed. In addition to normalization against one or multiple reference gene(s) and normalization against a reference sample (calibrator), a third normalization step against a base value has to be performed in a target-, sample- and condition specific manner.