发明名称 |
3'-DESOXYPENTOPYRANOSYL NUCLEIC ACID AND ITS PRODUCTION |
摘要 |
<p>Provided are processes for preparing a 3′-deoxypentopyranosyl oligomers with linkers for linking biomolecules. The processes can the steps of: bonding a 4′-protected-3′-deoxypentopyranosyl nucleoside to a solid support by coupling the 2′-OH group with a CPG support or other similar support with an amide linkage; deprotecting the 4′-protected-3′-deoxypentopyranosyl nucleoside at the 4′position; deprotecting the 4′-protected-3′-deoxypentopyranosyl nucleoside at the 4′position; and conjugating a linker to the free 4′position. The resulting product can be conjugated via the linker to a biomolecule. The method can include, prior to addition of the linker, reacting the 4′-OH group of the 4′-protected-3′-deoxypentopyranosyl nucleoside that is linked to the solid support with a 4′-protected-3′-deoxypentopyranosyl nucleoside phosphoramidite in the presence of a coupling reagent, and oxidizing the reaction product. This step can be repeated one or more times to produce an oligomer of desired length.</p> |
申请公布号 |
EP1105403(B1) |
申请公布日期 |
2012.04.04 |
申请号 |
EP19990945989 |
申请日期 |
1999.08.18 |
申请人 |
SANOFI |
发明人 |
MICULKA, CHRISTIAN;WAGNER, THOMAS;WINDHAB, NORBERT |
分类号 |
C07H19/04;C07H19/10;A61K31/70;A61K31/7042;A61K31/7052;A61K31/7064;A61K31/7068;A61K31/7072;A61K31/7076;A61P43/00;C07H19/20;C07H21/00;C12Q1/68;F16H15/42 |
主分类号 |
C07H19/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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