发明名称 |
Method for single oxygen atom incorporation into peptides |
摘要 |
Optimized enzymatic conditions incorporate a single oxygen atom into digested peptides using a peptidase. The incorporation of a single oxygen atom is especially useful for proteolytic 18O labeling in comparative proteomics. The optimized proteolytic 18O labeling minimizes the generation of a mixture of isotopic isoforms of the peptides resulting from incorporation of either one or two 18O atoms. The outcome is accurate quantification of isotopically labeled peptides. |
申请公布号 |
US2012077223(A1) |
申请公布日期 |
2012.03.29 |
申请号 |
US201113311404 |
申请日期 |
2011.12.05 |
申请人 |
MIYAGI MASARU;SEKHAR K. C.;UNIVERSITY OF NORTH DAKOTA |
发明人 |
MIYAGI MASARU;SEKHAR K. C. |
分类号 |
C12P21/00 |
主分类号 |
C12P21/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|