发明名称 |
COMPOUNDS AND METHODS FOR FRET BASED MEASUREMENT OF ENZYME ACTIVITY |
摘要 |
Provided are compositions and methods for Fluorescence/Forster Resonance Energy Transfer (FRET) analysis of cleavage of Von Willebrand Factor (VWF) polypeptides by the metalloprotease ADAMTS13. The polypeptides contain: i) a first fluorescent protein ii) a VWF amino acid sequence and iii) a second fluorescent protein. The VWF amino acid can contain any of a variety of amino acid substitutions. The method involves contacting a sample that contains ADAMTS13 and using FRET based measurements to determine ADAMTS13 cleavage of the recombinant polypeptide. |
申请公布号 |
WO2011153522(A3) |
申请公布日期 |
2012.03.29 |
申请号 |
WO2011US39232 |
申请日期 |
2011.06.06 |
申请人 |
THE RESEARCH FOUNDATION OF STATE UNIVERSITY OF NEW YORK;NEELAMEGHAM, SRIRAM;DAYANANDA, KANNAYAKANAHALLI |
发明人 |
NEELAMEGHAM, SRIRAM;DAYANANDA, KANNAYAKANAHALLI |
分类号 |
C07K19/00;C07K14/47;C12Q1/37 |
主分类号 |
C07K19/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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