METHOD OF ENHANCING EFFICIENCY AND SENSITIVITY IN POLYMERASE CHAIN REACTION AND COMPOSITION THEREFOR

摘要

PURPOSE: A method for enhancing PCR sensitivity and speed and a composition for the same are provided to quickly detect target microorganisms, cells or genes in a sample. CONSTITUTION: A method for enhancing PCR efficiency and speed comprises: a step of preparing a biological sample and PCR mixture and adding alkali to one or both; and a step of mixing the biological sample and PCR mixture and performing PCR. The biological sample includes cells, body fluid, or nucleic acids. The alkali is sodium hydroxide or potassium hydroxide. A composition for efficient PCR contains the PCR mixture and alkali.