METHOD FOR MANUFACTURING MULTILAYERED CELL SHEET, MULTILAYERED CELL SHEET HAVING VASCULAR NETWORK OBTAINED THEREBY, METHOD OF USE THEREOF
摘要
A method for manufacturing a multilayered cell sheet characterized in: fabricating a vascular bed that constructs a vascular network extending to the surface from a channel for perfusing a medium, the channel being embedded in a gel; and layering a cell sheet onto the vascular bed to construct a vascular network in the cell sheet. This manufacturing method makes it possible to construct a vascular network in the cell sheet and to fabricate a thick multilayered cell sheet in a simple manner by layering cell sheets. Such a thick multilayered cell sheet is useful as an in-vivo tissue substitute in regenerative medicine involving a variety of tissues.
申请公布号
WO2012036225(A1)
申请公布日期
2012.03.22
申请号
WO2011JP71056
申请日期
2011.09.14
申请人
TOKYO WOMEN'S MEDICAL UNIVERSITY;SAKAGUCHI, KATSUHISA;SHIMIZU, TATSUYA;SEKINE, HIDEKAZU;UMEZU, MITSUO;OKANO, TERUO