摘要 |
<P>PROBLEM TO BE SOLVED: To provide a fluorospectro-photometer for reducing influences by self-absorption without diluting a sample in fluorometry in a transmission direction of a high concentration sample where the influences by the self-absorption are problematic. <P>SOLUTION: The fluorescence spectrum in a reflection direction is measured in processing 1 and peak wavelength is detected in decision 1. When there is peak wavelength, on the ground that the sample has fluoresncence, identification wavelength is calculated from the fluorescence spectrum in the reflection direction obtained in the processing 1 in processing 2. Next, in processing 3, a transmission spectrum when an optical path length is changed is measured, and a cell having optical path length appropriate for measuring the fluorescence spectrum in the transmission direction is decided in decision 2. Finally, in processing 4, the fluorescence spectrum in the transmission direction in the cell having the optical path length accepted in the decision 2 is measured. <P>COPYRIGHT: (C)2012,JPO&INPIT |