发明名称 |
Method for Quantification of Small RNA Species |
摘要 |
The present invention relates to a method for amplification and quantification of microRNA molecules using quantitative reverse transcription polymerase chain reaction (qRT-PCR) technology. The method comprise the steps of (a) producing cDNA molecules complementary to microRNAs in a sample using polyadenylation and primer extension by reverse transcription, and (b) amplification and quantification of the cDNAs by qPCR using microRNA specific primer sets of forward and reverse primers containing LNA monomers. |
申请公布号 |
US2012071332(A1) |
申请公布日期 |
2012.03.22 |
申请号 |
US201013147562 |
申请日期 |
2010.02.02 |
申请人 |
BUSK PETER KAMP;EXIQON A/S |
发明人 |
BUSK PETER KAMP |
分类号 |
C12Q1/68;C07H21/02;C07H21/04;C12P19/34;C40B30/00 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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