METHOD AND DEVICE FOR ISOLATING CELLS FROM HETEROGENEOUS SOLUTION USING MICROFLUIDIC TRAPPING VORTICES
摘要
A method of isolating cells includes providing a microfluidic device having at least one microfluidic channel coupled to an inlet and an outlet, the at least one microfluidic channel comprises at least one expansion region disposed along the length thereof. The at least one expansion region is an abrupt increase in a cross-sectional dimension of the at least one microfluidic channel configured to generate a vortex within the at least one expansion region in response to fluid flow. A solution containing a population of cells at least some of which have diameters = 10 µm flows into the inlet. A portion of cells is trapped within vortex created within the at least one expansion region. The trapped cells may then released from the expansion region.
申请公布号
WO2012037030(A2)
申请公布日期
2012.03.22
申请号
WO2011US51224
申请日期
2011.09.12
申请人
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;DI CARLO, DINO;HUR, SOOJUNG, C.;MACH, ALBERT, J.