METHOD FOR SCREENING COMPOUNDS FOR TREATMENT OF TUMORS BEARING ACTIVATED ONCOGENES AND COMPOUNDS THEREOF

摘要

Method of screening for a compound for treatment of a tumor having at least one activated oncogene, wherein the compound is capable of increasing the expression of the at least one activated oncogene directly or indirectly acting on the untranslated regions of the mRNAs transcribed from the locus of said activated oncogene, the method comprising: i) contacting a compound with a cell comprising a nucleic acid construct, wherein the nucleic acid construct comprises a reporter gene coding sequence flanked by at least one untranslated region sequence taken from the mRNAs transcribed from the oncogene locus or segments thereof; and ii) detecting expression of a reporter polypeptide encoded by the reporter gene coding sequence; wherein an increase in the level of expression of the reporter polypeptide in the presence of the compound relative to the level of expression of the reporter polypeptide in absence of the compound indicates that the compound increases the dependent expression of the at least one activated oncogene directly or indirectly acting on the untranslated regions of the mRNAs transcribed from the oncogene locus of interest.