发明名称 |
A NOVEL HANSENULA POLYMORPHA GENE CODING FOR DOLICHYL-PHOSPHATE-MANNOSE DEPENDENT ALPHA-1,3-MANNOSYLTRANSFERASE AND PROCESS FOR THE PRODUCTION OF RECOMBINANT GLYCOPROTEINS WITH HANSENULA POLYMORPHA MUTANT STRAIN DEFICIENT IN THE SAME GENE |
摘要 |
The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using a Hansenula polymorpha system. In detail, the present invention relates to a process for producing a human-type glycoprotein by identifying a dolichyl-phosphate-mannose dependent ¥á-1,3-mannosyltransferase gene from H. polymorpha, constructing a H. polymorpha mutant strain producing a glycoprotein exhibiting reduced glycosylation by disrupting the identified gene, and subjecting the mutant strain to various genetic manipulations for the synthesis of human-type glycan. |
申请公布号 |
EP1941042(B1) |
申请公布日期 |
2012.03.21 |
申请号 |
EP20060812236 |
申请日期 |
2006.10.26 |
申请人 |
KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY |
发明人 |
KANG, HYUN AH;PARK, JEONG SEOK;KIM, MOO WOONG;KIM, EUN JUNG;MOON, HYE YUN;OH, DOO BYOUNG;HEO, JOO HYUNG;RHEE, SANG KI |
分类号 |
C12N15/54;C12N15/09;C12N15/31 |
主分类号 |
C12N15/54 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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