发明名称 |
GENETICALLY ENGINEERED PYRROLOQUINOLINE QUINONE DEPENDENT GLUCOSE DEHYDROGENASE COMPRISING AN AMINO ACID INSERTION |
摘要 |
The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known from Acinetobacter calcoaceticus, to genes encoding such variant s~GDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.
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申请公布号 |
CA2570593(C) |
申请公布日期 |
2012.03.20 |
申请号 |
CA20052570593 |
申请日期 |
2005.07.19 |
申请人 |
F. HOFFMANN-LA ROCHE AG |
发明人 |
BOENITZ-DULAT, MARA;LAGGERBAUER, JESSICA;SCHMUCK, RAINER;KRATZSCH, PETER;KNAPPE, WOLFGANG-REINHOLD |
分类号 |
C12N9/04;C12M1/40;C12N15/10;C12N15/53;C12Q1/32;G01N33/66 |
主分类号 |
C12N9/04 |
代理机构 |
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主权项 |
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地址 |
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