METHOD FOR HUMAN GENOTYPE DETERMINATION BY 4343 GENE BRCA1 (rsL799950) POSITION POLYMORPHISM

摘要

FIELD: medicine. ^ SUBSTANCE: invention may be used for human genotype determination by 4343 gene BRCA1 (rsl799950) position polymorphism The method consists in using allele-specific primers with real-time PCR values recording with using ¼éuorescence marked probes. A reaction mixture contains the primers different for each allele, the primer and the probe common for two alleles, and also the common fluorescent probe. ^ EFFECT: invention provides cutting expenses for genetic researches and making them more available due to the use of standard equipment and only one marked probe. ^ 2 dwg