<p>Methods are provided for ligating a 3' adapter and a 5' adapter to a target polynucleotide so as to avoid adapter dimer formation. Embodiments of the methods include adding a blocking oligonucleotide after the first ligation in which a 3' adapter is ligated to the target polynucleotide so that the blocking oligonucleotide is capable of hybridizing to excess 3' adapter and the ligated 3' adapter. Subsequently, a 5' adapter is ligated to the target polynucleotide thus avoiding adapter dimer formation.</p>
申请公布号
WO2012033687(A1)
申请公布日期
2012.03.15
申请号
WO2011US49972
申请日期
2011.08.31
申请人
NEW ENGLAND BIOLABS, INC.;MCREYNOLDS, LARRY, A.;MUNAFO, DANIELA