SCREENING-VERFAHREN MIT VERWENDUNG DES NEUEN SUBSTRATS EPHA4 FÜR GAMMA-SEKRETASE

摘要

The present invention provides a method of screening for compounds which affect the processing of EphA4 by ³-secretase, comprising the following steps:
(i) contacting a first biological composition containing ³-secretase or a biologically active fragment thereof with a second biological composition containing EphA4 in the presence and absence of a candidate compound;
(ii) measuring the cleavage of the EphA4 in the presence and absence of the candidate compound;
(iii) selecting those candidate compounds which affect the cleavage of the EphA4 by ³-secretase; and
(iv) identifying the candidate compounds selected in step (iii) as compounds which affect the processing of EphA4 by ³-secretase.