GENE OF COTTON EPSP SYNTHETASE VARIANT AND USES THEREOF

摘要

A cotton EPSP synthetase variant and artificial mutant gene MC-EPSPS encoding the EPSP synthetase variant are provided. The mutant gene encoding the variant with two mutant amino acid sites and anti-glyphosate property is obtained by designing mutant sites according to documents and analysis of protein structure and then conducting PCR amplification by using the EPSP synthetase gene of Gossypium hirsutum as template, which utilizes gene homologous recombination and multiple-site mutations of DNA sequence. The binding efficiency of the EPSP synthetase encoded by the mutant gene to glyphosate is decreased dominantly, and the EPSP synthetase has certain enzyme catalysis efficiency. The gene encoding the EPSP synthetase variant can be used to cultivating new breeds of transgenic plants with anti-glyphosate property.