发明名称 |
RECOMBINANT MICROORGANISM HAVING AN IMPROVED ABILITY TO PRODUCE BUTANOL AND A PRODUCTION METHOD FOR BUTANOL USING THE SAME |
摘要 |
The present invention relates to a recombinant microorganism having an improved ability to produce butanol and to a production method for butanol using the same, and more specifically relates to a recombinant microorganism for biosynthesis of butanol in which 2-ketoisovalerate, which is a precursor of L-valine, is used as an intermediate, and relates to a production method for butanol using the same. A feature of the recombinant microorganism according to the present invention is that a microorganism having the ability to produce 2-ketoisovalerate, which is a precursor of L-valine, has introduced therein: a gene coding for VorABC enzyme which is an enzyme that converts 2-ketoisovalerate into isobutyryl-CoA; a gene coding for an enzyme that converts isobutyryl-CoA into butyryl-CoA; a gene coding for an enzyme that converts butyryl-CoA into butyraldehyde; and a gene coding for an enzyme that converts butyraldehyde into butanol. Unlike existing Clostridium acetobutylicum, the recombinant microorganism according to the present invention is easy to grow, has a substantial strain-improvement potential due to additional metabolic flux manipulation and is useful as a microorganism for the industrial production of butanol, and a recombinant microorganism is provided which has an improved ability to produce butanol by simplifying the process for converting 2-ketoisovalerate to isobutyryl-CoA. |
申请公布号 |
WO2012033334(A2) |
申请公布日期 |
2012.03.15 |
申请号 |
WO2011KR06607 |
申请日期 |
2011.09.07 |
申请人 |
KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY;LEE, SANG YUP;CHOI, YONG JUN;PARK, JIN HWAN |
发明人 |
LEE, SANG YUP;CHOI, YONG JUN;PARK, JIN HWAN |
分类号 |
C12N1/21;C12N15/52;C12P7/16;C12R1/15 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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