| 摘要 |
The present invention has as an object to simplify the implant work in a series of the process from the separation of target cells by dielectrophresis to the cell cultivation and to realize a cell accumulation method that prevents bacteria contamination by bacteria. Method to achieve the objects The cell accumulation device includes the accumulation and cultivation vessel having a first electrode unit 16, an aggregation of the plural number of individual electrodes, allocated on the first substrate and a second electrode unit 17 allocated on the second substrate. The cell accumulation method has 1) a microbead trapping process, wherein microbeads 2 having biocompatibility are sent to the accumulation and cultivation vessel and AC voltage is applied to the first electrode unit and the second electrode unit to trap the microbeads on the individual electrodes and 2) a cell collection process, wherein after the microbead trapping process cellular suspension is sent into the inside of the vessel 101 of the accumulation and cultivation vessel 1 and AC voltage is applied to the first electrode unit and the second electrode unit to collect active cells 41 on said trapped microbeads.
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