Oxidoreductases for stereo-selective reduction of keto compounds

摘要

Method for enantioselective enzymatic reduction of a keto compound to its chiral hydroxy compound comprises reducing the keto compound by an oxidoreductase in the presence of a cofactor, where the oxidoreductase comprises an amino acid sequence. Method for the enantioselective enzymatic reduction of a keto compound to its chiral hydroxy compound comprises reducing the keto compound by an oxidoreductase in the presence of a cofactor, where the oxidoreductase comprises an amino acid sequence in which at least 70% of the amino acids are identical to the amino acids of one of the amino acid sequences comprising more than 26 amino acid (SEQ ID NO: 1 and 6) sequence and SEQ ID NO: 8 (not defined); at least 55% of the amino acids are identical to the amino acids of the amino acid sequence comprising fully defined more than 15 amino acids (SEQ ID NO: 2) sequences per fragment as given in the specification; at least 65% of the amino acids are identical to the amino acids of the fully defined more than 15 amino acid (SEQ ID NO: 3) sequence per fragment as give in the specification; at least 75% of the amino acids are identical to the amino acids of the fully defined more than 18 amino acids (SEQ ID NO: 4) sequences as given in the specification; at least 65% of the amino acids are identical to the amino acids of the fully defined more than 18 amino acids (SEQ ID NO: 5) sequences per fragment as given in the specification; at least 50% of the amino acids are identical to the amino acids of the he amino acid sequence (SEQ ID NO: 7) (not defined); or at least 72 % of the amino acids are identical to the amino acids of the amino acid sequence (SEQ ID NO: 129) (not defined). An independent claim is also included for an oxidoreductase used in the above procedure.