发明名称 |
A METHOD FOR EVALUATING THE ABILITY OF A COMPOUND TO INHIBIT THE PROTOPORPHYRINOGEN OXIDASE ACTIVITY |
摘要 |
The present invention provides: A method for evaluating the ability of a compound to inhibit the protoporphyrinogen oxidase activity, which comprises the steps of: (1) culturing a transformant expressing a protoporphyrinogen oxidase gene present in a DNA fragment in a medium containing substantially no protoheme compounds in each comparative system of the presence and absence of a test compound to measure a growth rate of the transformant under each condition, said transformant being resulted from a host cell deficient in the growing ability based on the protoporphyrinogen oxidase activity transformed with the DNA fragment in which a promoter functionable in the host cell and a protoporphyrinogen oxidase gene are operatively linked, and (2) determining the ability of the compound to inhibit the protoporphyrinogen oxidase activity by comparing the growth rates; and the like.
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申请公布号 |
CA2266807(C) |
申请公布日期 |
2012.03.13 |
申请号 |
CA19992266807 |
申请日期 |
1999.04.09 |
申请人 |
SUMITOMO CHEMICAL CO., LTD. |
发明人 |
SHIMOKAWATOKO, YASUTAKA;NISHIO, SHOICHI |
分类号 |
C12N15/53;A01H5/00;C12N9/02;C12Q1/02;C12Q1/26;C12Q1/68 |
主分类号 |
C12N15/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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