发明名称 |
Method of forming myeloid precursor cells from human embryonic stem cells |
摘要 |
This invention relates to the culture of dendritic cells from human embryonic stem (ES) cells. Human ES cells are first cultured into hematopoietic cells by co-culture with stromal cells. The cells now differentiated into the hematopoietic lineage are then cultured with GM-CSF to create a culture of myeloid precursor cells. Culture of the myeloid precursor cells with the cytokines GM-CSF and IL-4 causes functional dendritic cells to be generated. The dendritic cells have a unique phenotype, as indicated by their combination of cell surface markers.
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申请公布号 |
US8133732(B2) |
申请公布日期 |
2012.03.13 |
申请号 |
US20100876830 |
申请日期 |
2010.09.07 |
申请人 |
SLUKVIN IGOR I.;THOMSON JAMES A.;VODYANYK MAKSYM A.;GUMENYUK MARYNA E.;WISCONSIN ALUMNI RESEARCH FOUNDATION |
发明人 |
SLUKVIN IGOR I.;THOMSON JAMES A.;VODYANYK MAKSYM A.;GUMENYUK MARYNA E. |
分类号 |
C12N5/00;C12N5/02;C12N5/071;C12N5/0784 |
主分类号 |
C12N5/00 |
代理机构 |
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地址 |
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