NUCLEIC ACID FRAGMENTS FROM A RIBOSOMAL PROTEIN PROMOTER FOR ENHANCING GENE EXPRESSION
摘要
The present invention relates to nucleic acid fragments and constructs comprising genomic nucleotide sequences from the promoter region of a 60S ribosomal protein L32 gene (RPL32), for the production of a gene product of interest in a eukaryotic, preferably mammalian, host cell in the presence of a stringent selectable marker. The invention further relates to host cells comprising the nucleic acid constructs, to methods for generating the host cells and to methods for producing a gene product of interest using the host cells.
申请公布号
WO2012030218(A1)
申请公布日期
2012.03.08
申请号
WO2011NL50593
申请日期
2011.08.31
申请人
CELLAGENICS B.V.;OTTE, ARIE PIETER;HOEKSEMA, FEMKE;VERHEES, JOHN ANTONIUS;VAN BLOKLAND, HENRICUS JOHANNES MARIA
发明人
OTTE, ARIE PIETER;HOEKSEMA, FEMKE;VERHEES, JOHN ANTONIUS;VAN BLOKLAND, HENRICUS JOHANNES MARIA