NOVEL ENGINEERED MICROORGANISM PRODUCING SUCCINIC ACID WITH HIGH YIELD BY UTILIZING BOTH SUCROSE AND GLYCEROL AT THE SAME TIME AND METHOD FOR PREPARING SUCCINIC ACID USING THE SAME

摘要

PURPOSE: A mutant microorganism which produces succinic acid is provided to maximize fermentation yield and to minimize by-products. CONSTITUTION: A mutant microorganism uses sucrose and glycerol to produce succinic acid by weakening catabolic product suppressing metabolism of glycerol. The microorganism is lumen bacteria. The lumen bacteria are Mannheimia sp., Actinobacillus sp., or Anaerobiospirillum sp. Mannheimia sp. is Mannheimia succiniciproducens PALK(KCTC10973BP). A method for preparing succinic acid comprises: a step of culturing the mutant microorganism under anaerobic condition; and a step of collecting succinic acid from the culture liquid.