| 摘要 |
Provided is a method and growth chamber for the rapid and accurate detection of growth and metabolism of a cellular microorganism in isolation within one or a plurality of wells containing a population of microorganisms in a non-liquid, culture medium, wherein the cells are distributed at not greater than one cell per well at plating. Further provided is a gelled culture medium containing a non-toxic, water-soluble, phosphorescent compound which measures oxygen content (partial oxygen pressure) of a microorganism also contained therein, by oxygen-dependent quenching of phosphorescence; or the gel contains a fluorescent pH indicator that demonstrates growth of the microorganism by pH-dependent intensity change or wavelength shift in the emission spectrum. |
