FLOW-THROUGH PROTEIN PURIFICATION PROCESS

摘要

A process for isolating a protein of interest from a sample includes the steps of providing a sample comprising the protein of interest; and subjecting the sample to chromatography in flow-through mode, wherein the sample is applied to a chromatography material under conditions that highly bind the protein of interest but where the amount of protein of interest exceeds the binding capacity of the chromatography material such that a substantial amount of the protein of interest does not adsorb to the material; and collecting at least a portion of the sample that does not adsorb to the material, to thereby isolate the protein of interest.