发明名称 |
Sequence-specific detection of methylation in biomolecules |
摘要 |
A method for detecting sequence specific methylation in a biomolecule, comprising: (a) contacting the biomolecule with an S-adenosyl-L-methionine-dependent methyltransferase in the presence of a detectable cofactor of said methyltransferase; and (b) detecting whether the recognition sequence of said methyltransferase has been modified with the cofactor or a derivative thereof, wherein modification of the recognition sequence of said methyltransferase is indicative of an absence of methylation at said recognition sequence. Also disclosed is a cofactor specific for S-adenosyl-L-methionine-dependent methyltransferases, wherein said cofactor is an N-adenosylaziridine derivative with a reporter group attached to the 6 or 7 position of the adenine ring or attached to the aziridine ring. A complex of the cofactor and a methyltransferase a composition comprising the cofactor or the complex and the use of the cofactor or the complex for detecting sequence-specific methylation in DNA molecules are also disclosed. |
申请公布号 |
US8129106(B2) |
申请公布日期 |
2012.03.06 |
申请号 |
US20050570672 |
申请日期 |
2005.06.14 |
申请人 |
WEINHOLD ELMAR;MEIER THOMAS;DUEFEL HARTMUT;MARKERT-HAHN CHRISTINE;SCHMUCK RAINER;MAX-PLANCK-GESELISCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN E.V. |
发明人 |
WEINHOLD ELMAR;MEIER THOMAS;DUEFEL HARTMUT;MARKERT-HAHN CHRISTINE;SCHMUCK RAINER |
分类号 |
C12Q1/68;C07H15/00;C07H19/04;C07H19/16;C07H21/04;C12Q1/70;G01N33/53 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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