DETECTING CHROMOSOMAL REARRANGEMENT

摘要

Provided herein are methods of detecting a chromosomal rearrangement in a subject. The methods comprise isolating deoxyribonucleic acid (DNA) comprising a target DNA sequence from a biological sample from the subject; performing a multiplex PCR assay comprising at least five primer pairs to the targeted DNA sequence, wherein the multiplex PCR assay produces a plurality of labeled nucleic acid sequences; contacting an array with the plurality of labeled nucleic acid sequences; and detecting a binding pattern of the labeled nucleic acid sequences to the array. The binding pattern indicates the presence or absence of a chromosomal rearrangement in the subject.