发明名称 |
METHODS, WORKFLOWS, KITS, APPARATUSES, AND COMPUTER PROGRAM MEDIA FOR NUCLEIC ACID SAMPLE PREPARATION FOR NUCLEIC ACID SEQUENCING |
摘要 |
A method for preparing a nucleic acid sample for nucleic acid sequencing includes amplifying a nucleic acid target sequence using a primer bound to a first capture substrate; capturing an amplified nucleic acid product by the first capture substrate; generating at least one sequencing ladder from the amplified nucleic acid product using at least one sequencing primer; capturing the at least one sequencing ladder by hybridizing the at least one sequencing ladder to a complementary capture compound on a second capture substrate; and removing the at least one sequencing ladder from the second capture substrate. The first and/or second capture substrate may include a magnetic particle. Other methods, workflows, kits, and computer program media for nucleic acid sample preparation are also disclosed. |
申请公布号 |
WO2012006116(A3) |
申请公布日期 |
2012.03.01 |
申请号 |
WO2011US42240 |
申请日期 |
2011.06.28 |
申请人 |
LIFE TECHNOLOGIES CORPORATION;BREVNOV, MAXIM;BOLCHAKOVA, ELENA;FURTADO, MANOHAR |
发明人 |
BREVNOV, MAXIM;BOLCHAKOVA, ELENA;FURTADO, MANOHAR |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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