发明名称 |
METHOD FOR PRODUCTION OF cDNA LIBRARY HAVING REDUCED CONTENT OF cDNA CLONE DERIVED FROM HIGHLY EXPRESSED GENE |
摘要 |
<p>A method for efficiently constructing a cDNA library having a reduced content of cDNA clones derived from a highly expressed gene is provided. According to the method for constructing a cDNA library using a double-stranded DNA primer having oligo dT and mRNA as a template, the proportion of cDNA clones derived from a highly expressed gene in a cDNA library was decreased through coexistence with a probe having a property of binding to the mRNA of the highly expressed target gene so as to inhibit a cDNA extension reaction resulting from reverse transcriptase.</p> |
申请公布号 |
EP2322612(A4) |
申请公布日期 |
2012.02.29 |
申请号 |
EP20090809882 |
申请日期 |
2009.08.25 |
申请人 |
HITACHI HIGH-TECHNOLOGIES CORPORATION;JAPAN AS REPRESENTED BY DIRECTOR GENERAL OF NATIONAL REHABILITATION CENTER FOR PERSONS WITH DISABILITIES |
发明人 |
OHTOKO, KUNIYO;SUGIYAMA MASAHIDE;KATO SEISHI |
分类号 |
C12N15/10;C40B40/06;C40B50/06 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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