| 摘要 |
The invention relates to derivatives of the Calcium Dependant Antibiotic variants CDA 1 b, CDA2a, CDA3a, CDA3b, CDA3b CDA4a, CDA4b, CDA2d, CDA2fb, CDA2fa, CDA5a and CDA6a which comprise either a 2,3-epoxybutanoyl fatty acid side-chain or a butanoyl fatty acid side-chain, in place of the wild-type CDA trans 2,3 epoxyhexanoyl fatty acid side-chain. The invention also relates to a method of synthesizing derivatives of the Calcium Dependant Antibiotic variants which comprise either a 2,3- epoxybutanoyl fatty acid side-chain or a butanoyl fatty acid side-chain in place of the wild-type CDA fatty acid trans 2,3 epoxyhexanoyl, comprising mutating a bacterial strain comprising the CDA biosynthetic gene cluster and so as to mutate fabF3 of the CDA biosynthetic gene cluster so that residue Phe107 of FabF3 is altered to either an isoleucine, a leucine or a valine residue. |
