发明名称 |
Hybridization chain reaction amplification for in situ imaging |
摘要 |
The present invention relates to the use of fluorescently labeled nucleic acid probes to identify and image analytes in a biological sample. In the preferred embodiments, a probe is provided that comprises a target region able to specifically bind an analyte of interest and an initiator region that is able to initiate polymerization of nucleic acid monomers. After contacting a sample with the probe, labeled monomers are provided that form a tethered polymer. Triggered probes and self-quenching monomers can be used to provide active background suppression. |
申请公布号 |
US8124751(B2) |
申请公布日期 |
2012.02.28 |
申请号 |
US20100790379 |
申请日期 |
2010.05.28 |
申请人 |
PIERCE NILES A.;DIRKS ROBERT;FRASER SCOTT E.;CALIFORNIA INSTITUTE OF TECHNOLOGY |
发明人 |
PIERCE NILES A.;DIRKS ROBERT;FRASER SCOTT E. |
分类号 |
C07H21/04;C12Q1/68 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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